Title: IL-3 mediated enhancement of particulate antigen presentation by macrophages
Authors:James P. Moran, E.M. Lord, K-Y Yeh, E. Storozynsky, J. Frelinger
Periodical:Journal of Immunotherapy, v21, n3, 1998, p205-210
Abstract:Mice were exposed to interleukin- (IL-) 3 in vivo by injection of tumor cells transfected with the IL-3 gene. At 10 days post tumor injection, bone marrow cells were recovered, pulsed with particulate antigen in the form of ovalbumin (Ova)-coated magnetic beads, and tested for their ability to present antigen via class I to an Ova/class I-restricted T cell hybridoma.
Title: Alteration of tumor response to radiation by interleukin-2 gene transfer
Authors:James P. Moran, P.C. Keng, B.F. Fenton, C.J. Koch, E.M. Lord
Periodical:British Journal of Cancer, v82, n4, 2000, p937-944
Abstract:We have previously shown that BALB/c-derived EMT6 mammary tumours transfected with interleukin (IL)-2 have decreased hypoxia compared to parental tumours, due to increased vascularization. Since hypoxia is a critical factor in the response of tumours to radiation treatment, we compared the radiation response of IL-2-transfected tumours to that of parental EMT6 tumours.
Title:Transfection of the genes for interleukin-12 into the K1735 melanoma and the EMT6 mammary sarcoma murine cell lines reveals distinct mechanisms of anti-tumor activity
Authors:James P. Moran, S.A. Gerber, C. Martin, J.G. Frelinger, E.M. Lord
Periodical:International Journal of Cancer, v106, n5, 2003, p690-698
Abstract:Interleukin 12 (IL-12) is a pleiotropic cytokine with multiple effects on the immune system. The antitumor effects of locally produced IL-12 were examined in 2 tumor model systems. IL-12 expressing EMT6 mammary sarcomas (EMT6/IL-12) grew temporarily and then regressed resulting in mice that were immune to a further challenge of EMT6 cells. Interestingly, the IL-12 expressing K1735 melanomas (K1735/IL-12) maintained a lag phase of nonmeasurable growth for several weeks, followed by tumor outgrowth that was associated with a loss of IL-12 production. Tumor-infiltrating lymphocytes (TILs) isolated from EMT6/IL-12 tumors effectively lysed EMT6 target cells, whereas K1735/IL-12 TILs lacked lytic activity.
Title:Mechanism of IL-12 mediated alterations in tumour blood vessel morphology analysis using whole-tissue mounts
Authors:James P. Moran, S.A. Gerber, J.G. Frelinger, J.A. Frelinger, B.F. Fenton, E.M. Lord
Periodical:British Journal of Cancer, v88, n9, 2003, p1453-1461
Abstract:New blood vessel formation within tumours is a critical feature for tumour growth. A major limitation in understanding this complex process has been the inability to visualise and analyse vessel formation. Here, we report on the development of a whole-tissue mount technique that allows visualisation of vessel structure. Mice expressing green fluorescent protein (GFP) made it possible to easily see GFP+ vessels within non-GFP-expressing B16 melanoma tumours.
Title:Characterization of a lymph node within the mouse prostate: detailed analysis using whole mount history
Authors:James P. Moran, S.A. Gerber, M.J. Turner, A.A. Lugade, J.G. Frelinger, E.M. Lord
Periodical:Prostate, v63, n2, 2005, p105-116
Abstract:BACKGROUND: Due to the prevalence of prostate disease, there is a growing interest in the immunobiology of the prostate and its contribution to such pathologies. Further study is needed to fully characterize immune responses within the prostate. METHODS: Mouse ventral prostates were removed and analyzed using conventional immunohistochemistry, or by a novel whole mount technique, which allows the visualization of complete structures within the prostate. RESULTS: A lymphoid structure was detected within the base of the mouse ventral prostate. Whole mount and cryostat sections revealed an organized arrangement of lymphocytes, along with dendritic cells interspersed throughout the node. It possessed a rich network of lymph node specific high endothelial venules (HEVs) and adjoining lymphatics containing immune cells. CONCLUSIONS: Our results demonstrate the presence of a lymphoid structure within the base of the mouse ventral prostate possessing traits characteristic of an organized peripheral lymph node.
Title:Local radiation therapy of B16 melanoma tumors increases the generation of tumor antigen-specific effector cells that traffic to the tumor
Authors:James P. Moran, A.A. Lugade, S.A. Gerber, R.C. Rose, J.G. Frelinger, E.M. Lord
Periodical:Journal of Immunology, v174, n12, 2005, p7516-7523
Abstract:Immunotherapy of cancer is attractive because of its potential for specificity and limited side effects. The efficacy of this approach may be improved by providing adjuvant signals and an inflammatory environment for immune cell activation. We evaluated antitumor immune responses in mice after treatment of OVA-expressing B16-F0 tumors with single (15 Gy) or fractionated (5 x 3 Gy) doses of localized ionizing radiation.
Title:Radiation induced IFN-y production within the tumor microenvironment influences anti-tumor immunity
Authors:James P. Moran, A.A. Lugade, E.W. Sorensen, S.A. Gerber, J.G. Frelinger, E.M. Lord
Periodical:Journal of Immunology,
v180, n5, 2008, p3132-3139
Abstract:Alterations to the tumor microenvironment following localized irradiation may influence the effectiveness of subsequent immunotherapy. The objective of this study was to determine how IFN-? influences the inflammatory response within this dynamic environment following radiotherapy.
Title:Bifidobacterium animalis causes extensive duodenitis and mild colonic inflammation in monoassociated interleukin-10 deficient mice
Authors:James P. Moran, J. Walter, S.L. Tonkonogy, G.W. Tannock, R.B. Sartor
Periodical:Inflammatory Bowel Disease, v15, n7, 2009, p1022-1031
Abstract:We recently showed that Bifidobacterium animalis is more prevalent within the colons of IL-10 deficient (-/-) mice than in wild type (WT) animals colonized with the same specific pathogen free (SPF) fecal contents. Here we tested the ability of this organism to cause T cell-mediated intestinal inflammation by introducing it into germ-free (GF) IL-10-/- mice.
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